Table 4

List of genes that were differentially expressed between the mammary glands of rats exposed to a low versus high fat n-3 PUFA diet prepubertally, P < 0.05

Gene IDAccession no.Unigene no.Gene nameLN3/HN3Cell cycle proliferationApoptosisDifferentiation
Up-regulated genes
1237AI059137Rn.8737Myr3 for myosin I heavy chain (Myo1e)78.1497
2476AA859285Rn.2661Macrophage migration inhibitory factor (Mif)77.9136xx
922AI071262Rn.11349Trans-Golgi network integral membrane protein (Tgoln1)64.705
1208AA998830Rn.11204Brain expressed myelocytomatosis oncogene (Bmyc)51.2979
1539AA998895Rn.3539Nucleoplasmin-related protein (nuclear protein B23)50.9449
1191AA997450Rn.34521G-protein–coupled receptor kinase interactor 1 (GIT1)49.9268
2195AA955902Rn.9493Myogenic differentiation 1 (Myod1)45.0848x
475AA956941Rn.10450New England Deaconess E-box binding factor42.5661
575AI070721Rn.6281Glial cell line–derived neurotrophic factor receptor α (Gfra4)37.5564
821AA956736Rn.2432Fyn proto-oncogene (Fyn)35.4852x
1136AA923854Rn.3446unc-50 homologue (Unc50)31.3974
1833AA901147Rn.11305Atrophin-1 (Atn1)28.9757x
142AA963308Rn.40942Latent transforming growth factor β binding protein 1 (Ltbp1)26.7251
1809AA874828Rn.10962FBJ osteosarcoma oncogene B (Fosb)26.1847xx
1498AI111925Rn.25905Carboxypeptidase D (Cpd)20.7826
1808AA875109Rn.6977Pleiomorphic adenoma gene-like 1 (Plagl1)17.6601xxx
2470AA875142Rn.7615HT3 receptor17.012
731AA817997Rn.1214Ribosomal protein L2410.9046
447AA924772Rn.11325Metallothionein-III (Mt3)9.3542x
2455AA858723Rn.2366Acyl-CoA synthetase long-chain family member 1 (Acsl1)8.5659
402AA900189Rn.2140Cell division cycle 37 homologue (cdc37)7.6244x
2147AA874884Rn.3160Heme oxygenas (Hmox)7.5387xx
1582AI044828Rn.967Thioredoxin nuclear gene encoding mitochondrial protein6.7203
214AI070507Rn.8778Fibromodulin (Fmod)6.025
1464AA874962Rn.3189Nucleoporin 210 (Nup210)5.3968
1158AA955301Rn.2342Mannoside acetylglucosaminyltransferase (mgat2)5.3782
785AA901316Rn.7223DCoH gene5.0508
2584AI045303Rn.13808Coronin 7 (Coro 7)4.2594
2246AI045830Rn.35934Aconitase 1 (Aco1)4.2058
231AI136065Rn.32973Arrestin, β2 (Arbb2)3.9432x
242AA866277Rn.3036Guanine nucleotide binding protein (G protein), α inhibiting 2 (Gnai2)3.7385x
2101AA818096Rn.241Quinoid dehydropteridine reductase (Qdpr)3.1367
2466AA875135Rn.2803ADP-ribosylation factor-like 5A (Arl5a)2.9366
813AA926255Rn.3520Meprin 1β (Mep1b)2.9173
510AA998213Rn.10248A kinase (PRKA) anchor protein 8 (Akap8)2.6375x
859AA998607Rn.11133Aminoadipate aminotransferase (Aadat)2.5544
2141AA900555Rn.31745N-ethylmaleimide sensitive fusion protein attachment protein α (Napa)2.4051x
1767AA819242Rn.1692Glutathione synthetase (Gss)2.3836
208AI070102Rn.8653Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, β polypeptide (Ywhab)2.37
2186AA955550Rn.6686Cytochrome c oxidase subunit (Cox5b)2.2624
5006AA900426Rn.2778Ubiquitin-conjugating enzyme E2D (UBC415 homologue, yeast; Ubc2d3)2.1154
4956AA899334Rn.2661Macrophage migration inhibitory factor (Mif)2.096
146AA965204Rn.4037Complement component 1, s subcomponent (C1s)2.0794x
1129AA923894Rn.6477Tclone42.0759
1800AA874973Rn.40118Nuclear protein E3-32.0512
827AA956889Rn.1904Adenylate cyclase 4 (Adcy4)2.0485
385AA818858Rn.1463Peptidylprolyl isomerase A (Ppia)1.96
455AA925731Rn.3561Isocitrate dehydrogenase 1 (NADPH), soluble (Idh1)1.9171
2207AA957519Rn.555Stathmin 1 (Stmn1)1.9014xx
4861AA818749Rn.1457Nuclear transcription factor-Y γ (Nfyc)1.8462
837AA964578Rn.28Calpactin I heavy chain1.796
1469AA899597Rn.484Ribosomal protein L18 (Rpl18)1.7523
4567AA900379Rn.22304HLA-B–associated transcript 3 (BAT3)1.6102xx
1477AA924274Rn.4206Ribosomal protein L22 (Mrpl22)1.5434
1218AI043796Rn.9686Solute carrier family 18 A2 (vesicular monoamine), member 2 (Slc18a2)1.5382
794AA924727Rn.2953Collagen, type 1, α1 (Co1a1)1.4963
216AI070517Rn.10421Sensory neuron synuclein1.486
818AI112794Rn.11014Dynein cytoplasmic 1 intermediate chain 2 (Dync1;2)1.4444
140AA963258Rn.6282Insulin-like growth factor I (IGF-I)1.3394xx
2450AA858866Rn.401325'-Nucleotidase ecto (Nt5e)1.3057
143AA963506Rn.32904Endoplasmic reticulum protein 29 (Erp29)1.2564
1096AA819420Rn.2042Ras homologue gene family, member B (Rhob)1.2352xx
2481AA874917Rn.783Biglycan (Bgn)1.2301
5237AA858896Rn.2464Fetuin β (Fetub)1.1707
832AA964162Rn.10838Sphingomyelin phosphodiesterase 3 (Smpd3)1.0946x
1150AI111917Rn.10524Solute carrier family 16 (monocarboxylic acid transporters), member 7 (Slc16a7)1.0614
Down-regulated genes
1880AA964350Rn.32984Natriuretic peptide receptor 2 (Npr2)0.0736
5030AA924006Rn.40532Tissue inhibitor of metalloproteinase 3 (Timp3)0.1238
1928AI045770Rn.11281Calcium channel, voltage-dependent, P/Q type, α 1A subunit (Cacna1a)0.1877
2586AI043606Rn.3729Chymotrypsinogen B1 (Ctrb1)0.2197
487AA963451Rn.11366Opioid binding protein/cell adhesion molecule-like (Opcml)0.2347x
2579AI029586Rn.9781Cholecystokinin (CCK)0.2914x
2462AA875115Rn.2491Endothelial differentiation, sphingolipid G-protein–coupled receptor 5 (Edg5)0.37
1818AA901195Rn.11080Hydroxymethylbilane synthase (Hmbs)0.3706
2514AA925476Rn.32253Coiled-coil domain containing 56 (Ccdc56)0.3891
217AI070783Rn.31889RAB 3A interacting protein (Rab3ip)0.3982
526AI030725Rn.13361PDZ and LIM domain 3 (Pdlim3)0.3989
1463AA875174Rn.2816Ras-related GTP binding A (Rraga)0.4141xx
2618AI146173Rn.2618ATP synthase, H+ transporting mitochondrial F1 complex, β subunit (Atp5b)0.4163
2607AI059029Rn.10379Glycine receptor, α2 subunit (Glra2)0.4208xx
2453AA858888Rn.2458Tubulin, β5 (Tubb5)0.4327
2611AI070064Rn.10066Aquaporin 5 (Aqp5)0.4341
2204AA957589Rn.11365Erythropoietin (Epo)0.4557x
1897AI028940Rn.11267Vascular cell adhesion molecule 1 (Vcam1)0.4765
2544AA965091Rn.10071Protein kinase N1 (PKN1)0.5002
2780AA819300Rn.33239Squalene epoxidase (Sqle)0.5081
223AI071126Rn.10253Cardiotrophin-1 (Ctf1)0.5094x
812AA926359Rn.25722Receptor-linked protein tyrosine phosphatase (PTP-P1)0.5498
2483AA874919Rn.3174MutS homologue 2 (E. coli; Msh2)0.573xxx
1147AA924911Rn.11103α cardiac myosin heavy chain0.7165x

NOTE: Bolded genes were confirmed via real-time PCR.