Table 1.

Concentration of nicotine, cotinine, and trans-3′-hydroxycotinine in the urine of nicotine consuming micea

Nicotine, ng/mLCotinine, ng/mLTrans-3′-hydroxycotinine, ng/mL
GroupTreatmentbTimeMeanRangeMeanRangeMeanRange
2Nicotine (0–46 wks)/saline1–2 wksc (n = 26)1,360 ± 1,04065–3,5701,260 ± 1,240116–4,70012,200 ± 9,0101,600–34,000
5 mo (n = 14)1,270 ± 1,170217–3,6204,400 ± 3,200450–10,60027,900 ± 9,82017,100–49,600
4Nicotine (0–46 wks)/NNK1–2 wks (n = 24)1,900 ± 2,55053–7,7004,790 ± 5,86070–17,50018,900 ± 13,5004,300–48,000
5 mo (n = 15)2,170 ± 2,760156–10,5005,700 ± 4,300930–16,00042,900 ± 19,40016,600–74,400
5Nicotine (0–2 wks)/NNKa1–2 wks (n = 25)980 ± 93022–2,8703,520 ± 6,220150–3,12017,000 ± 10,5002,920–37,500
5 monddndnd
6Nicotine (2–46 wks)/NNKb1–2 wksndndnd
5 mo (n = 20)840 ± 71462–2,5605,910 ± 4,140820–14,70037,800 ± 19,4009,750–83,400
  • aUrine obtained from individual mice was analyzed by LC/MS-MS, values are provided for 2 time points for individual mice from which urine was collected.

  • bThe protocol is as illustrated in Fig. 1: Beginning at 5 weeks of age, female A/J mice (groups 2, 4, and 5) were provided 0.44 μmol/mL nicotine in the drinking water; after 2 weeks, animals in groups 4, 5, and 6 were administered a single dose of NNK (10 μmol), nicotine was discontinued for group 5 and initiated in group 6 at the time of the NNK injection.

  • cUrine was collected from a single mouse between 7 and 14 days of nicotine consumption and the remaining samples were from a single collection per mouse from 2 to 7 days.

  • dnd, not determined, animals were not consuming nicotine at this time.