Skip to main content
  • AACR Publications
    • Blood Cancer Discovery
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

AACR logo

  • Register
  • Log in
  • My Cart
Advertisement

Main menu

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Meeting Abstracts
    • Collections
      • COVID-19 & Cancer Resource Center
      • "Best of" Collection
      • Editors' Picks
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • Editors' Picks
    • OnlineFirst
    • Citation
    • Author/Keyword
    • RSS Feeds
    • My Alert Summary & Preferences
  • News
    • Cancer Discovery News
  • COVID-19
  • Webinars
  • Search More

    Advanced Search

  • AACR Publications
    • Blood Cancer Discovery
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

User menu

  • Register
  • Log in
  • My Cart

Search

  • Advanced search
Cancer Prevention Research
Cancer Prevention Research
  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Meeting Abstracts
    • Collections
      • COVID-19 & Cancer Resource Center
      • "Best of" Collection
      • Editors' Picks
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • Editors' Picks
    • OnlineFirst
    • Citation
    • Author/Keyword
    • RSS Feeds
    • My Alert Summary & Preferences
  • News
    • Cancer Discovery News
  • COVID-19
  • Webinars
  • Search More

    Advanced Search

Research Article

Oral Epithelium as a Surrogate Tissue for Assessing Smoking-Induced Molecular Alterations in the Lungs

Manisha Bhutani, Ashutosh Kumar Pathak, You-Hong Fan, Diane D. Liu, J. Jack Lee, Hongli Tang, Jonathan M. Kurie, Rodolfo C. Morice, Edward S. Kim, Waun Ki Hong and Li Mao
Manisha Bhutani
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ashutosh Kumar Pathak
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
You-Hong Fan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Diane D. Liu
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
J. Jack Lee
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hongli Tang
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jonathan M. Kurie
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rodolfo C. Morice
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Edward S. Kim
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Waun Ki Hong
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Li Mao
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1158/1940-6207.CAPR-08-0058 Published June 2008
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • Additional Files
  • Fig. 1
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 1

    A flexible bronchoscope is inserted through the nose or mouth to examine the airways after i.v. sedation (bottom right). Bronchial brushings are taken from six predetermined sites (top right). Oral brushing using a cytologic brush is done (top left) by rubbing the inner side of the left cheek (bottom left). From Visual Art © 2008, The University of Texas, M. D. Anderson Cancer Center; with permission.

Tables

  • Figures
  • Additional Files
  • Table 1

    Gene promoter methylation status at baseline using patient (combining six bronchial brush sites within each patient) as an analysis unit

    Characteristicp16FHITp16/FHIT
    BronchialOralBronchialOralBronchialOral
    MUMUMUMUMUMU
    No. patients (%)74 (58.3)53 (41.7)24 (19.4)100 (80.4)50 (39.4)77 (60.6)19 (15.3)105 (84.7)98 (77.2)29 (22.8)39 (31.2)86 (68.8)
    Age (y), n (%)
     <5462 (48.8)36 (58.1)26 (41.9)12 (20.3)47 (79.7)24 (38.7)38 (61.3)9 (15.3)50 (84.7)46 (74.2)16 (25.8)19 (31.7)41 (68.3)
     ≥5465 (51.2)38 (58.5)27 (41.5)12 (18.5)53 (81.5)26 (40)39 (60)10 (15.4)55 (84.6)52 (80)13 (20)20 (30.8)45 (69.2)
    P0.960.790.880.980.440.91
    Sex, n (%)
     F58 (45.7)39 (67.2)19 (32.8)12 (21.1)45 (78.9)25 (43.1)33 (56.9)10 (17.9)46 (82.1)49 (84.5)9 (15.5)20 (35.1)37 (64.9)
     M69 (54.3)35 (50.7)34 (49.3)12 (17.9)55 (82.1)25 (36.2)44 (63.8)9 (13.2)59 (86.8)49 (71)20 (29)19 (27.9)49 (72.1)
    P0.060.660.430.480.070.39
    Smoker, n (%)
    Current99 (78)59 (59.6)40 (40.4)21 (21.9)75 (78.1)38 (38.4)61 (61.6)12 (12.5)84 (87.5)77 (77.8)22 (22.2)31 (32)66 (68)
    Former28 (22)15 (53.6)13 (43.4)3 (10.7)25 (89.3)12 (42.9)16 (57.1)7 (25)21 (75)21 (75)7 (25)8 (28.6)20 (71.4)
    P0.560.280.670.110.760.73
    Pack-years, n (%)
     <3036 (28.3)12 (33.3)24 (66.7)3 (8.6)32 (91.4)14 (38.9)22 (61.1)6 (17.1)29 (82.9)20 (55.6)16 (44.4)9 (25)27 (75)
     30-4032 (25.2)23 (71.9)9 (28.1)6 (18.8)26 (81.3)15 (46.9)17 (53.1)4 (12.5)28 (87.5)29 (90.6)3 (9.4)9 (28.1)23 (71.9)
     41-5024 (18.9)15 (62.5)9 (37.5)5 (20.8)19 (79.2)8 (33.3)16 (66.7)6 (25)18 (75)19 (79.2)5 (20.8)10 (41.7)14 (58.3)
     ≥5035 (27.6)24 (68.6)11 (31.4)10 (30.3)23 (69.7)13 (37.1)22 (62.9)3 (9.1)30 (90.9)30 (85.7)5 (14.3)11 (33.3)22 (66.7)
    P*0.00030.080.940.720.00030.34
    Quit-years, n (%)
     <199 (78)59 (59.6)40 (40.4)21 (21.9)75 (78.1)38 (38.4)61 (61.6)12 (12.5)84 (87.5)77 (77.8)22 (22.2)31 (32)66 (68)
     1-1018 (14.2)9 (50)9 (50)3 (16.7)15 (83.3)9 (50)9 (50)6 (33.3)12 (66.7)14 (77.8)4 (22.27 (38.9)11 (61.1)
     >1010 (7.9)6 (60)4 (40)0 (0)10 (100)3 (30)7 (70)1 (10)9 (90)7 (70)3 (30)1 (10)9 (90)
    P†10.210.7410.690.17

    Abbreviations: M, methylated; U, unmethylated.

    • ↵*Comparing methylation between the groups <30 and ≥30 pack-years.

    • ↵†Comparing methylation between the groups <10 and ≥10 quit-years.

  • Table 2

    Association between oral methylation status and bronchial methylation status

    BronchialOral
    p16FHITp16/FHIT
    UMUMUM
    U49*2761272
    M512229185937
    P†0.0002<0.00010.001
    • ↵*Data are expressed as number of patients.

    • ↵†Fisher's exact test.

  • Table 3

    Correlation between oral methylation status and bronchial methylation index

    Bronchial methylation index
    OralnMean ± SD, median (range)P*
    Baseline
     p16U1000.18 ± 0.22, 0.17 (0-0.83)<0.0001
    M240.47 ± 0.30, 0.50 (0-1.00)
     FHITU1050.10 ± 0.20, 0.00 (0-1.00)<0.0001
    M190.50 ± 0.29, 0.50 (0-1.00)
     p16/FHITU860.27 ± 0.26, 0.17 (0-1.00)<0.0001
    M390.53 ± 0.29, 0.50 (0-1.00)
    3 mo
     p16U890.14 ± 0.21, 0.00 (0-1.00)<0.0001
    M360.39 ± 0.29, 0.33 (0-1.00)
     FHITU1130.08 ± 0.18, 0.00 (0-1.00)<0.0001
    M140.57 ± 0.28, 0.67 (0.17-1.00)
     p16/FHITU830.21 ± 0.24, 0.17 (0-1.00)<0.0001
    M440.50 ± 0.31, 0.50 (0-1.00)
    • ↵*Wilcoxon's rank-sum test comparing bronchial brush methylation index between oral brush methylation groups.

  • Table 4

    Comparison of modulation of promoter methylation status between oral and bronchial epithelia after 3-mo intervention

    Oral baseline/3 monBronchial methylation index (baseline/3 mo)
    Mean ± SD, median (range)P*
    p16
     M/M7−0.16 ± 0.19, −0.17 (−0.5-0)0.003
     M/U16−0.15 ± 0.40, −0.17 (−0.67-0.67)
     U/M280.16 ± 0.34, 0.17 (−0.50-1.00)
     U/U71−0.05 ± 0.28, 0 (−0.83-0.67)
    FHIT
     M/M3−0.11 ± 0.25, −0.17 (−0.33-0.17)<0.0001
     M/U16−0.46 ± 0.30, −0.50 (−0.83-0)
     U/M100.42 ± 0.33, 0.42 (−0.17-1.00)
     U/U950.01 ± 0.22, 0 (−0.50-1.00)
    p16/FHIT
     M/M16−0.06 ± 0.37, −0.17 (−0.83-0.67)0.007
     M/U23−0.22 ± 0.38, −0.33 (−0.83-0.50)
     U/M270.16 ± 0.37, 0.17 (−0.50-1.00)
     U/U59−0.05 ± 0.31, 0 (−0.83-0.50)
    • ↵*Kruskal-Wallis test comparing bronchial brush methylation index modulation among oral brush methylation modulation groups.

Additional Files

  • Figures
  • Tables
  • Perspective

    The following Perspective is available for this article:

    The Oral Cavity as a Molecular Mirror of Lung Carcinogenesis
    David Sidransky
    PreviousNext
    Back to top
    Cancer Prevention Research: 1 (1)
    June 2008
    Volume 1, Issue 1
    • Table of Contents
    • Table of Contents (PDF)
    • About the Cover

    Sign up for alerts

    View this article with LENS

    Open full page PDF
    Article Alerts
    Sign In to Email Alerts with your Email Address
    Email Article

    Thank you for sharing this Cancer Prevention Research article.

    NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

    Enter multiple addresses on separate lines or separate them with commas.
    Oral Epithelium as a Surrogate Tissue for Assessing Smoking-Induced Molecular Alterations in the Lungs
    (Your Name) has forwarded a page to you from Cancer Prevention Research
    (Your Name) thought you would be interested in this article in Cancer Prevention Research.
    CAPTCHA
    This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
    Citation Tools
    Oral Epithelium as a Surrogate Tissue for Assessing Smoking-Induced Molecular Alterations in the Lungs
    Manisha Bhutani, Ashutosh Kumar Pathak, You-Hong Fan, Diane D. Liu, J. Jack Lee, Hongli Tang, Jonathan M. Kurie, Rodolfo C. Morice, Edward S. Kim, Waun Ki Hong and Li Mao
    Cancer Prev Res June 1 2008 (1) (1) 39-44; DOI: 10.1158/1940-6207.CAPR-08-0058

    Citation Manager Formats

    • BibTeX
    • Bookends
    • EasyBib
    • EndNote (tagged)
    • EndNote 8 (xml)
    • Medlars
    • Mendeley
    • Papers
    • RefWorks Tagged
    • Ref Manager
    • RIS
    • Zotero
    Share
    Oral Epithelium as a Surrogate Tissue for Assessing Smoking-Induced Molecular Alterations in the Lungs
    Manisha Bhutani, Ashutosh Kumar Pathak, You-Hong Fan, Diane D. Liu, J. Jack Lee, Hongli Tang, Jonathan M. Kurie, Rodolfo C. Morice, Edward S. Kim, Waun Ki Hong and Li Mao
    Cancer Prev Res June 1 2008 (1) (1) 39-44; DOI: 10.1158/1940-6207.CAPR-08-0058
    del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
    • Tweet Widget
    • Facebook Like
    • Google Plus One

    Jump to section

    • Article
      • Abstract
      • Materials and Methods
      • Results
      • Discussion
      • Disclosure of Potential Conflicts of Interest
      • Footnotes
      • References
    • Figures & Data
    • Info & Metrics
    • PDF
    Advertisement

    Related Articles

    Cited By...

    More in this TOC Section

    • Obesity and Telomere Length in Prostate Stromal Cells
    • Regulatory T Cells and Melanocytic Nevi
    • mt-sDNA Specificity in 45–49 Year-Olds
    Show more Research Articles
    • Home
    • Alerts
    • Feedback
    • Privacy Policy
    Facebook   Twitter   LinkedIn   YouTube   RSS

    Articles

    • Online First
    • Current Issue
    • Past Issues

    Info for

    • Authors
    • Subscribers
    • Advertisers
    • Librarians

    About Cancer Prevention Research

    • About the Journal
    • Editorial Board
    • Permissions
    • Submit a Manuscript
    AACR logo

    Copyright © 2021 by the American Association for Cancer Research.

    Cancer Prevention Research
    eISSN: 1940-6215
    ISSN: 1940-6207

    Advertisement