Human Papillomavirus (HPV) 16/18 E6 Oncoprotein Expression in Infections with Single and Multiple Genotypes

Zeni Wu; Ting-Yuan Li; Mingyue Jiang; Lulu Yu; Jing Zhao; Hairui Wang; Xun Zhang; Wen Chen; Youlin Qiao

doi:10.1158/1940-6207.CAPR-18-0343

DOI: 10.1158/1940-6207.CAPR-18-0343 Published February 2019

Article Figures & Data

Tables

Table 1.

Sociodemographics and risk factors for study population (n, %)

			HPV16 (n = 621)			HPV18 (n = 94)
	Total (n = 1,781)	HPV Positive (n = 1,069)	Single infection (n = 424)	Multiple infection (n = 197)	P	Single infection (n = 44)	Multiple infection (n = 50)	P
Age, mean (range)	48.2 (20–75)	48.1 (20–75)	47.1 (23–69)	48.5 (27–75)		49.5 (31–68)	48.4 (25–67)
<30	56 (3.1)	41 (3.8)	17 (4.0)	3 (1.5)	0.100	0 (0.0)	2 (4.0)	0.598
30 ∼ 39	249 (14.0)	166 (15.5)	71 (16.7)	35 (17.8)		5 (11.4)	5 (10.0)
40 ∼ 49	674 (37.8)	371 (34.7)	159 (37.5)	71 (36.0)		15 (34.1)	21 (42.0)
50 ∼ 59	569 (31.9)	341 (31.9)	135 (31.8)	56 (28.4)		19 (43.2)	18 (36.0)
≥60	233 (13.1)	150 (14.0)	42 (9.9)	32 (16.2)		5 (11.4)	4 (8.0)
Marital status
Married/cohabitation	1,764 (99.0)	1,055 (98.7)	416 (98.1)	193 (98.0)	0.904	44 (100.0)	50 (100.0)	—–
Divorced/widowed	17 (1.0)	14 (1.3)	8 (1.9)	4 (2.0)		0 (0.0)	0 (0.0)
Smoking
No	1,737 (97.5)	1,034 (96.7)	402 (94.8)	191 (97.0)	0.203	41 (93.2)	50 (100.0)	0.061
Yes	44 (2.5)	35 (3.3)	20 (4.7)	4 (2.0)		3 (6.8)	0 (0.0)
Drinking
No	1,430 (80.3)	797 (74.6)	330 (77.8)	144 (71.6)	0.236	36 (81.8)	40 (80.0)	0.823
Yes	351 (19.7)	272 (25.4)	91 (21.5)	54 (27.4)		8 (18.2)	10 (20.0)
Gravidity
≤2	627 (35.2)	360 (33.7)	134 (31.6)	67 (34.0)	0.551	16 (36.4)	14 (28.0)	0.385
≥3	1,154 (64.8)	709 (66.3)	290 (68.4)	130 (66.0)		28 (63.6)	36 (72.0)
Parity
≤2	1,257 (70.6)	725 (67.8)	289 (68.2)	136 (69.0)	0.827	31 (70.5)	38 (76.0)	0.544
≥3	524 (29.4)	344 (32.2)	135 (31.8)	61 (31.0)		13 (29.5)	12 (24.0)
Oral contraceptive
No	1,773 (99.6)	1,062 (99.5)	422 (99.5)	196 (99.5)	0.952	43 (97.7)	50 (100.0)	0.284
Yes	8 (0.4)	5 (0.5)	2 (0.5)	1 (0.5)		1 (2.3)	0 (0.0)
Menopause
No	1,131 (63.5)	655 (61.3)	273 (64.4)	123 (62.4)	0.638	25 (56.8)	31 (62.0)	0.609
Yes	650 (36.5)	414 (38.7)	151 (35.6)	74 (37.6)		19 (43.2)	19 (38.0)

Table 2.

Distribution of histology and presence of E6 oncoprotein in HPV16/18 DNA–positive women (E6 protein/DNA, %)

	Normal/CIN1	CIN2/3	SCC/ADC	Total
HPV16 (E6 protein/DNA, %)	11/75 (14.7)	85/166 (51.2)	335/380 (88.2)	431/621 (69.4)
Single HPV16 infections	6/42 (14.3)	51/100 (51.0)	260/282 (92.2)	317/424 (74.8)
Multiple HPV16 infections	5/33 (15.2)	34/66 (51.5)	75/98 (76.5)	114/197 (57.9)
HPV16 & 18	0/5 (0.0)	0/3 (0.0)	12/20 (60.0)	12/28 (42.9)
HPV16 & 52	2/9 (22.2)	7/14 (50.0)	15/16 (93.8)	24/39 (61.5)
HPV16 & 59/56/66	0/10 (0.0)	14/21 (66.7)	17/23 (73.9)	31/54 (57.4)
HPV16 & 51	1/8 (12.5)	4/5 (80.0)	7/8 (87.5)	12/21 (57.1)
HPV16 & 33/58	2/11 (18.2)	8/21 (38.1)	22/28 (78.6)	32/60 (53.3)
HPV16 & 39/68/35	1/13 (7.7)	9/15 (60.0)	11/14 (78.6)	21/42 (50.0)
HPV16 & 31	0/6 (0.0)	4/7 (57.1)	9/13 (69.2)	13/26 (50.0)
HPV16 & 45	0/4 (0.0)	0/2 (0.0)	1/4 (25.0)	1/10 (10.0)
HPV18 (E6 protein/DNA, %)	9/26 (34.6)	3/6 (50.0)	49/62 (79.0)	61/94 (64.9)
Single HPV18 infections	3/9 (33.3)	1/2(50.0)	31/33 (93.9)	35/44 (79.5)
Multiple HPV18 infections	6/17 (35.3)	2/4 (50.0)	18/29 (62.1)	26/50 (52.0)
HPV16 & 18	1/5 (20.0)	1/3 (50.0)	10/20 (50.0)	12/28 (42.9)
HPV18 & 52	2/2 (100.0)	1/1 (100.0)	1/3 (33.3)	4/6 (66.7)
HPV18 & 59/56/66	3/7 (42.9)	0/0 (0.0)	3/5 (60.0)	6/12 (60.0)
HPV18 & 31	2/3 (66.7)	0/0 (0.0)	1/2 (50.0)	3/5 (60.0)
HPV18 & 39/68/35	1/6 (16.7)	0/0 (0.0)	4/4 (100.0)	5/10 (50.0)
HPV18 & 45	0/0 (0.0)	0/0 (0.0)	1/2 (50.0)	1/2 (50.0)
HPV18 & 33/58	3/6 (50.0)	0/0 (0.0)	1/3 (33.3)	4/9 (44.4)
HPV18 & 51	0/4 (0.0)	0/0 (0.0)	1/2 (50.0)	1/6 (16.7)