Article Figures & Data
Figures
- Fig. 1
Raloxifene-induced cytotoxicity determined by trypan blue assay on HCT-116 (ER-β positive) and DLD (ER-β negative) cells. Cells were exposed to 2.0 to 10.0 μmol/L of raloxifene for 24 h. IC50 is calculated based on 50% inhibition of cell proliferation at a specific concentration of raloxifene. Points, mean of three independent experiments; bars, SE. *, P value. Values are significantly different from control by unpaired two-tailed t test (*, P < 0.106; **, P < 0.007-0019; ***, P < 0.0001) near the points. DLD cell line was observed to be more resistant compared with HCT-116 cell line.
- Fig. 2
A and C, protein expression levels of PCNA and ER-β were carried out by Western immunoblotting from the protein extracts of HCT-116 cells treated with various concentration of raloxifene. B and D, immunocytochemistry for PCNA and ER-β expression in raloxifene-treated and untreated HCT-116 cells. Intense positive nuclear staining (brown stain) is observed in control cells with diminished/spotty staining in treated HCT-116 cells. E, immunohistochemical analysis of ER-β protein expression in rat colon adenocarcinomas and normal colonic mucosa. An intense positive staining (brown stain) is observed in tumor tissue compared with normal colonic mucosa.
- Fig. 3
A, up-regulation of ER-β protein expression in AOM-induced colonic adenocarcinomas in comparison with normal mucosa by Western immunoblotting. B, up-regulation of ER-β mRNA expression in AOM-induced colonic adenocarcinomas in comparison with normal mucosa by RT-PCR. Statistical “P value” is shown as a bar graph adjacent to each figure.
- Fig. 4
A, effect of raloxifene on AOM-induced total mean number of colonic ACF formation in F344 rats. B, effect of raloxifene on AOM-induced colonic four or more aberrant crypt formation in F344 rats.
Volume 2, Issue 1
