Article Figures & Data
Figures
- Figure 1.
Effect of unmodified combinations of ASP, FA and Ca (G3–G7) on the AOM-induced aberrant crypts in the rat colon. Statistical 2-way ANOVA analysis followed by a Bonferroni post hoc analyses for aberrant crypts distribution according to the position on colon (Set 1; *, P < 0.05; **, P < 0.001). AOM, azoxymethane.
- Figure 2.
Dose response curve from Set 1 and probit analysis (inset) to calculate the effective dose (ED50) for the range of doses used (G3–G7).
- Figure 3.
Effect of (A) unmodified combinations of ASP, FA and Ca (G10–G12) compared to the (B) modified NP combinations of chemopreventive agents (G13–G15). Statistical 2-way ANOVA analysis followed by a Bonferroni post hoc analyses for ACF distribution according to the position on colon (*, P < 0.05; **, P < 0.001)
- Figure 4.
Histochemical H&E stains of longitudinal sections of rat distal colon depicting the nuclear proliferation of cancer cells when observed under a light microscope (×40) for the control (G8–G9) and NP-treated groups (G13–G15).
- Figure 5.
Effect of individual doses of NP-mediated single chemopreventive agent (G18–GS20) and that of a combined dose (G21) on ACF inhibition in the distal colon of rat. Statistical 2-way ANOVA analyses followed by a Bonferroni post hoc analysis for aberrant crypts distribution according to the position on colon (*, P < 0.05)
Tables
- Table 1.
Treatment plan showing groups of Sprague-Dawley rats treated with unmodified and nanoparticle forms of chemopreventive agents aspirin, folic acid, and calcium at various doses
Set Group (n = 6) Treatment plan Dose, mg/kg Set 1 (unmodified combination) G1 Saline (control) 0 G2 AOM (control) 0 G3 ASP + FA + Ca 400 + 32 + 650 G4 ASP + FA + Ca 133 + 11 + 216 G5 ASP + FA + Ca 40 + 3.2 + 65 G6 ASP + FA + Ca 13 + 1.1 + 22 G7 ASP + FA + Ca 4 + 0.32 + 6.5 Set 2 (unmodified and modified combination) G8 Saline (control) 0 G9 AOM (control) 0 G10 ASP + FA + Ca 133 + 11 + 216 G11 ASP + FA + Ca 80 + 6.4 + 130 G12 ASP + FA + Ca 40 + 3.2 + 65 G13 ASP (NP) + FA (NP) + Ca 133 + 11 + 216 G14 ASP (NP) + FA (NP) + Ca 80 + 6.4 + 130 G15 ASP (NP) + FA (NP) + Ca 40 + 3.2 + 65 Set 3 (modified combination) G16 Saline (control) 0 G17 AOM (control) 0 G18 ASP (NP) 40 G19 FA (NP) 3.2 G20 Ca 65 G21 ASP (NP) + FA (NP) + Ca 40 + 3.2 + 65 Abbreviations: ASP, aspirin; AOM, azoxymethane; FA, folic acid; Ca, calcium; NP, nanoparticles.
Supplementary Data
Files in this Data Supplement:
- Supplementary Figure A - PDF file - 120K
Volume 4, Issue 10
