Skip to main content
  • AACR Publications
    • Blood Cancer Discovery
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

AACR logo

  • Register
  • Log in
  • My Cart
Advertisement

Main menu

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Meeting Abstracts
    • Collections
      • COVID-19 & Cancer Resource Center
      • "Best of" Collection
      • Editors' Picks
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • Editors' Picks
    • OnlineFirst
    • Citation
    • Author/Keyword
    • RSS Feeds
    • My Alert Summary & Preferences
  • News
    • Cancer Discovery News
  • COVID-19
  • Webinars
  • Search More

    Advanced Search

  • AACR Publications
    • Blood Cancer Discovery
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

User menu

  • Register
  • Log in
  • My Cart

Search

  • Advanced search
Cancer Prevention Research
Cancer Prevention Research
  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Meeting Abstracts
    • Collections
      • COVID-19 & Cancer Resource Center
      • "Best of" Collection
      • Editors' Picks
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • Editors' Picks
    • OnlineFirst
    • Citation
    • Author/Keyword
    • RSS Feeds
    • My Alert Summary & Preferences
  • News
    • Cancer Discovery News
  • COVID-19
  • Webinars
  • Search More

    Advanced Search

Epidemiology/Lifestyle Factors

Abstract A101: Urinary isothiocyanate excretion profiles in response to acute feeding of various cruciferous vegetables in humans

Sandi L. Navarro, Hannah Frenkel, Wendy K. Thomas, Karen Makar, C.Y. Wang and Johanna W. Lampe
Sandi L. Navarro
Fred Hutchinson Cancer Research Center, Seattle, WA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hannah Frenkel
Fred Hutchinson Cancer Research Center, Seattle, WA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Wendy K. Thomas
Fred Hutchinson Cancer Research Center, Seattle, WA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Karen Makar
Fred Hutchinson Cancer Research Center, Seattle, WA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
C.Y. Wang
Fred Hutchinson Cancer Research Center, Seattle, WA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Johanna W. Lampe
Fred Hutchinson Cancer Research Center, Seattle, WA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1158/1940-6207.PREV-12-A101 Published November 2012
  • Article
  • Info & Metrics
Loading

Abstract

Higher consumption of cruciferous vegetables is associated with lower risk of several cancers. Isothiocyanates (ITC), the bioactive compounds derived from glucosinolates in cruciferous vegetables, are thought to exert chemoprotective effects through modulation of several pathways critical to carcinogenesis. Urinary ITC excretion is used in epidemiologic studies as a measure of cruciferous vegetable intake; however, considerable inter-individual variation in ITC excretion is observed. Several factors may influence glucosinolate metabolism and excretion, including gut microbial hydrolysis and genetic variation in human enzymes. Thus, intake is not necessarily equivalent to ITC exposure. In a randomized cross-over trial, we evaluated total urinary ITC excretion in healthy participants (n=12 men; n=13 women) in response to 100 μmol glucosinolate intake from three acute feedings of cooked cruciferous vegetables– watercress (67 g), broccoli (125 g), and cabbage (250 g). The vegetables were fed alongside a standardized frozen pasta meal (227 g). GSTM1 genotyping (present or null) was conducted on buccal cell DNA. Total urinary ITC excretion (dithiocarbamates plus glutathione-derived conjugates) was assayed by HPLC from 24 h urine collections adjusted for completeness by creatinine. Linear mixed models were used to assess the effects of dietary exposure and urinary ITC excretion. Mean urinary ITC excretion was 7.21 (95% CI: 5.81, 8.94); 8.35 (95% CI: 6.73, 10.35); and 10.08 (95% CI: 8.20, 12.41) μmol/24 h for watercress, cabbage and broccoli, respectively. As a group, participants excreted significantly more ITC after consumption of broccoli compared to watercress (P=0.01). Intra- and inter-individual variances across all 3 vegetables were 0.29 and 0.07, respectively, and the intra-class correlation (ICC) was 0.20 when evaluating the three dietary measures as replicates of 100 μmol glucosinolate intakes. There were no main effects of GSTM1 genotype (n=9 positive; n=16 null) or ethnicity (n=14 Caucasians; n=9 Asians). Urinary ITC excretion profiles significantly differed between broccoli and watercress, as well as within and between individuals (ICCs below 0.45 indicate poor correlation between repeated measures). This suggests that ITC bioavailability within individuals differs by type of crucifer consumed. ITC are involved in the modulation of several pathways involved in carcinogenesis, therefore an individual's actual exposure to ITC may affect their ultimate risk of cancer.

Citation Format: Sandi L. Navarro, Hannah Frenkel, Wendy K. Thomas, Karen Makar, C.Y. Wang, Johanna W. Lampe. Urinary isothiocyanate excretion profiles in response to acute feeding of various cruciferous vegetables in humans. [abstract]. In: Proceedings of the Eleventh Annual AACR International Conference on Frontiers in Cancer Prevention Research; 2012 Oct 16-19; Anaheim, CA. Philadelphia (PA): AACR; Cancer Prev Res 2012;5(11 Suppl):Abstract nr A101.

  • ©2012 American Association for Cancer Research.
PreviousNext
Back to top
Cancer Prevention Research: 5 (11 Supplement)
November 2012
Volume 5, Issue 11 Supplement
  • Table of Contents

Sign up for alerts

Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Cancer Prevention Research article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Abstract A101: Urinary isothiocyanate excretion profiles in response to acute feeding of various cruciferous vegetables in humans
(Your Name) has forwarded a page to you from Cancer Prevention Research
(Your Name) thought you would be interested in this article in Cancer Prevention Research.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Abstract A101: Urinary isothiocyanate excretion profiles in response to acute feeding of various cruciferous vegetables in humans
Sandi L. Navarro, Hannah Frenkel, Wendy K. Thomas, Karen Makar, C.Y. Wang and Johanna W. Lampe
Cancer Prev Res November 1 2012 (5) (11 Supplement) A101; DOI: 10.1158/1940-6207.PREV-12-A101

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Abstract A101: Urinary isothiocyanate excretion profiles in response to acute feeding of various cruciferous vegetables in humans
Sandi L. Navarro, Hannah Frenkel, Wendy K. Thomas, Karen Makar, C.Y. Wang and Johanna W. Lampe
Cancer Prev Res November 1 2012 (5) (11 Supplement) A101; DOI: 10.1158/1940-6207.PREV-12-A101
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
Advertisement

Related Articles

Cited By...

More in this TOC Section

Epidemiology/Lifestyle Factors

  • Abstract PR12: Adolescent inflammation and body mass index in relation to colorectal cancer risk
  • Abstract PL05-01: Obesity, IGF-1 and cancer prevention: Mechanistic insights from transdisciplinary studies
  • Abstract PL04-03: Evidence for increased muscle breakdown associated with early pancreatic cancer
Show more Epidemiology/Lifestyle Factors

Diet and Cancer

  • Abstract PL03-01: A gnotobiotic mouse model demonstrates that dietary fiber protects against colorectal tumorigenesis in a microbiota- and butyrate-dependent manner
  • Abstract CN10-03: Childhood obesity and leukemia: Opportunities for intervention
  • Abstract PL02-03: Diet, nutrition, and cancer: Past, present, and future
Show more Diet and Cancer

Diet and Cancer: Poster Presentations - Proffered Abstracts

  • Abstract PL03-01: A gnotobiotic mouse model demonstrates that dietary fiber protects against colorectal tumorigenesis in a microbiota- and butyrate-dependent manner
  • Abstract CN10-03: Childhood obesity and leukemia: Opportunities for intervention
  • Abstract PL02-03: Diet, nutrition, and cancer: Past, present, and future
Show more Diet and Cancer: Poster Presentations - Proffered Abstracts
  • Home
  • Alerts
  • Feedback
  • Privacy Policy
Facebook   Twitter   LinkedIn   YouTube   RSS

Articles

  • Online First
  • Current Issue
  • Past Issues

Info for

  • Authors
  • Subscribers
  • Advertisers
  • Librarians

About Cancer Prevention Research

  • About the Journal
  • Editorial Board
  • Permissions
  • Submit a Manuscript
AACR logo

Copyright © 2021 by the American Association for Cancer Research.

Cancer Prevention Research
eISSN: 1940-6215
ISSN: 1940-6207

Advertisement