Skip to main content
  • AACR Publications
    • Blood Cancer Discovery
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

AACR logo

  • Register
  • Log in
  • My Cart
Advertisement

Main menu

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Meeting Abstracts
    • Collections
      • COVID-19 & Cancer Resource Center
      • "Best of" Collection
      • Editors' Picks
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • Editors' Picks
    • OnlineFirst
    • Citation
    • Author/Keyword
    • RSS Feeds
    • My Alert Summary & Preferences
  • News
    • Cancer Discovery News
  • COVID-19
  • Webinars
  • Search More

    Advanced Search

  • AACR Publications
    • Blood Cancer Discovery
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

User menu

  • Register
  • Log in
  • My Cart

Search

  • Advanced search
Cancer Prevention Research
Cancer Prevention Research
  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Meeting Abstracts
    • Collections
      • COVID-19 & Cancer Resource Center
      • "Best of" Collection
      • Editors' Picks
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • Editors' Picks
    • OnlineFirst
    • Citation
    • Author/Keyword
    • RSS Feeds
    • My Alert Summary & Preferences
  • News
    • Cancer Discovery News
  • COVID-19
  • Webinars
  • Search More

    Advanced Search

Chemoprevention and Biological Therapies

Abstract B56: Activation of Chk1 by Piperine causes cell cycle arrest and apoptosis in melanoma cells

Neel Fofaria and Sanjay K. Srivastava
Neel Fofaria
Texas Tech University Health Sciences Center, Amarillo, TX.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sanjay K. Srivastava
Texas Tech University Health Sciences Center, Amarillo, TX.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1158/1940-6207.PREV-12-B56 Published November 2012
  • Article
  • Info & Metrics
Loading

Abstract

The National Cancer Institute estimates that about 76,250 new cases of melanomas will be diagnosed in 2012 in the United States leading to at least 9810 related deaths. Therefore, novel strategies to control and treat melanoma are of prime importance. In this study, we determined the cytotoxic effects of piperine, a major constituent of black (Piper nigrum Linn) and long pepper (Piper longum Linn), in melanoma cell lines. Piperine treatment inhibited the growth of SKMEL-28 and B16-F0 cells in a dose and time-dependent manner. The growth inhibitory effects of piperine were mediated by cell cycle arrest of both the cell lines in G1 phase. The G1 arrest by piperine correlated with the down-regulation of cyclin D1 and induction of p21. Furthermore, this growth arrest was associated with DNA damage caused by piperine treatment as indicated by phosphorylation of H2A.X at Ser139, activation of ataxia telangiectasia and Rad3-related protein (ATR) and checkpoint kinase 1 (Chk1). Pretreatment with AZD 7762, a Chk1 inhibitor not only abrogated the activation of Chk1 but also piperine mediated G1 arrest. Furthermore, transfection of cells with Chk1 siRNA completely protected the cells from G1 arrest induced by piperine treatment. The G1 arrest by piperine was also confirmed with the down-regulation of E2F1 and decreased phosphorylation of retinoblastoma protein (Rb), indicating the inhibition of the dynamic transcription complex. In addition, apoptosis induced by piperine was associated with down-regulation of XIAP and Bid (uncleaved) and cleavage of Caspase-3 and PARP. In addition, our results showed that piperine treatment generated ROS in melanoma cells. Blocking ROS generation by Tiron prevented the cells from piperine mediated cell cycle arrest. Taken together, our results demonstrate that activation of Chk1 is required for piperine induced G1 phase cell cycle arrest. [Supported in part by R01 grants CA 106953 and CA 129038 awarded to S.K.S by National Cancer Institute, NIH]

Citation Format: Neel Fofaria, Sanjay K. Srivastava. Activation of Chk1 by Piperine causes cell cycle arrest and apoptosis in melanoma cells. [abstract]. In: Proceedings of the Eleventh Annual AACR International Conference on Frontiers in Cancer Prevention Research; 2012 Oct 16-19; Anaheim, CA. Philadelphia (PA): AACR; Cancer Prev Res 2012;5(11 Suppl):Abstract nr B56.

  • ©2012 American Association for Cancer Research.
PreviousNext
Back to top
Cancer Prevention Research: 5 (11 Supplement)
November 2012
Volume 5, Issue 11 Supplement
  • Table of Contents

Sign up for alerts

Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Cancer Prevention Research article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Abstract B56: Activation of Chk1 by Piperine causes cell cycle arrest and apoptosis in melanoma cells
(Your Name) has forwarded a page to you from Cancer Prevention Research
(Your Name) thought you would be interested in this article in Cancer Prevention Research.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Abstract B56: Activation of Chk1 by Piperine causes cell cycle arrest and apoptosis in melanoma cells
Neel Fofaria and Sanjay K. Srivastava
Cancer Prev Res November 1 2012 (5) (11 Supplement) B56; DOI: 10.1158/1940-6207.PREV-12-B56

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Abstract B56: Activation of Chk1 by Piperine causes cell cycle arrest and apoptosis in melanoma cells
Neel Fofaria and Sanjay K. Srivastava
Cancer Prev Res November 1 2012 (5) (11 Supplement) B56; DOI: 10.1158/1940-6207.PREV-12-B56
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
Advertisement

Related Articles

Cited By...

More in this TOC Section

Chemoprevention and Biological Therapies

  • Abstract PR03: Targeting multiple cell cycle regulatory points for the prevention of triple-negative breast cancer
  • Abstract PR05: Low dose aspirin that reduces mortality from lung adenocarcinoma inhibits both platelet COX-1 and the biosynthesis of PGE2
  • Abstract PR13: Circadian/melatonin disruption by dim light at night drive chemotherapy resistance in breast cancer
Show more Chemoprevention and Biological Therapies

New Molecular Targets/Mechanisms of Drug Action

  • Abstract PR-01: Properties of adenomas in subjects treated with difluoromethylornithine and sulindac for reduction of colorectal adenomas
  • Abstract B85: Role of the TASK2 in regulating breast cancer cell proliferation
  • Abstract B106: Inhibition of IKKb and NFκB signaling by a novel curcumin analogue
Show more New Molecular Targets/Mechanisms of Drug Action

New Molecular Targets/Mechanisms of Drug Action: Poster Presentations - Proffered Abstracts

  • Abstract PR-01: Properties of adenomas in subjects treated with difluoromethylornithine and sulindac for reduction of colorectal adenomas
  • Abstract B85: Role of the TASK2 in regulating breast cancer cell proliferation
  • Abstract B106: Inhibition of IKKb and NFκB signaling by a novel curcumin analogue
Show more New Molecular Targets/Mechanisms of Drug Action: Poster Presentations - Proffered Abstracts
  • Home
  • Alerts
  • Feedback
  • Privacy Policy
Facebook   Twitter   LinkedIn   YouTube   RSS

Articles

  • Online First
  • Current Issue
  • Past Issues

Info for

  • Authors
  • Subscribers
  • Advertisers
  • Librarians

About Cancer Prevention Research

  • About the Journal
  • Editorial Board
  • Permissions
  • Submit a Manuscript
AACR logo

Copyright © 2021 by the American Association for Cancer Research.

Cancer Prevention Research
eISSN: 1940-6215
ISSN: 1940-6207

Advertisement