Article Figures & Data
Figures
- Figure 1.
Methylation status of 14 promoter CpG islands and LINE1 in healthy young individuals (Young), cancer-free subjects (Control), and gastric cancer training set (gastric cancer) groups examined by bisulfite pyrosequencing. Horizontal bars represent mean methylation values. Methylation levels in whole blood DNA between gastric cancer and CONTROL were compared using the Student t test and the logistic regression model was used to adjust for age when the P values were less than 0.05.
- Figure 2.
Methylation status of 3 promoter CpG islands in healthy young individuals (Young), cancer-free subjects (Control), and gastric cancer training set (gastric cancer) groups examined by qMSP. Horizontal bars represent mean methylation values. The relative level of methylated DNA is depicted as 40-dCt [Ct of specific gene—Ct of mC-LESS (internal control)]. A higher 40-dCt represents more methylation of the target biomarker. Methylation levels in whole blood DNA between gastric cancer and CONTROL were compared using the Student t test.
- Figure 3.
Age-related methylation. A, methylation status of 8 promoter CpG islands and LINE1 in relation to aging. The healthy young individuals, control, and gastric cancer training set groups were all included in this analysis. Statistical analyses were conducted using the Spearman correlation analysis. B, mean Z score of the methylation levels of 9 age-related loci in relation and aging. The healthy young individuals (young subjects), control, and gastric cancer training set groups (control patients and patients with gastric cancer) were all included in this analysis. Statistical analyses were conducted using the Spearman correlation analysis. Left is a scatter plot of all the data by age, whereas right is a bar graph of all the patients divided by study group.
- Figure 4.
Relative telomere length examined by quantitative real-time PCR. A, telomere length in healthy young individuals (Young), cancer-free subjects (Control), and gastric cancer training set (gastric cancer) groups. Horizontal bars represent mean telomere length. B, telomere length, in relation to age. C, telomere length in relation to the mean Z score of the methylation levels of 9 age-related loci. Statistical analyses were conducted using the Student t test (A) and Spearman correlation analysis (B, C).
- Figure 5.
Methylation status of SFRP1 in cancer-free subjects (CONTROL) and the gastric cancer validation set. Horizontal bars represent mean methylation values. Methylation levels in whole blood DNA between gastric cancer and CONTROL were compared using the Student t test and the logistic regression model was used to adjust for age.
Tables
- Table 2.
SFRP1 methylation status and clinicopathologic subtypes of gastric cancer
Variables (n) SFRP1 methylation (mean ± SEM%) Gender Male (110) 11.4 ± 0.26 Female (48) 11.6 ± 0.31 H. pylori status Negative (38) 11.2 ± 0.46 Positive (120) 11.5 ± 0.23 Histology Intestinal (110) 11.5 ± 0.26 Diffuse (48) 11.3 ± 0.31 Location Antrum (49) 11.9 ± 0.43 Body (81) 11.2 ± 0.25 Cardia (28) 11.4 ± 0.48 Staging Early (82) 11.8 ± 0.33 Advanced (76) 11.2 ± 0.23 Morphology Elevated or protruding (40) 11.8 ± 0.44 Depressed or with ulceration (105) 11.3 ± 0.25 Scirrhous (13) 11.1 ± 0.55 NOTE: Methylation status was not determined for 5 cases.
Supplementary Data
Files in this Data Supplement:
- Supplementary Figures 1 and 2 - PDF - 96K, Supplementary Fig. 1: Methylation status of 16 CpG island promoters and LINE1 in 8 primary gastric cancer tissues and 6 healthy gastric mucosae. Methylation was measured by bisulfite-pyrosequencing. Horizontal bars represent mean methylation values. Supplementary Fig. 2: Association between relative telomere length and methylation status for 4 promoter CpG islands. Statistical analyses were performed using the Spearman correlation analysis.
- Supplementary Figure Legends; Supplementary Tables 1-3 - PDF - 86K, Supplementary Table 1. Primer sequences used in pyrosequencing. Supplementary Table 2. Primer and probe sequences used in qMSP. Supplementary Table 3. Primer sequences used in telomere length measurement.
Volume 6, Issue 10
