Table 1.

Significantly greater inflammation, proinflammatory molecule levels, and aromatase expression were found in both the MG and visceral fat (VF) of ob/ob compared with wild-type mice.

EndpointMGVF
Wild-typeob/obPWild-typeob/obP
Inflammatory foci0.8 (0.0, 2.6)13.8 (8.5, 20.4)<0.0010 (0.0,10.6)64.5 (25.5, 202)<0.001
Relative TNF-α expression0.9 (0.6, 4.9)4.9 (0.7, 9.7)0.0071.0 (0.5, 2.8)5.3 (0.6, 8.7)0.02
Relative IL-1β expression1.0 (0.4, 2.3)2.9 (0.3, 9.7)0.021.1 (0.03, 6.0)5.7 (1.1, 7.9)0.005
Relative Cox-2 expression1.0 (0.4, 2.5)3.8 (1.2, 5.7)0.0011.0 (0.4, 3.7)2.2 (0.6, 4.8)0.02
Relative aromatase expression1.2 (0.2, 5.6)6.1 (0.04, 7.6)0.0070.9 (0.4, 2.7)1.8 (0.8, 6.8)0.009
Aromatase activity90 (68,112)210 (146, 278)<0.00198 (67, 154)272 (177, 355)<0.001

NOTE: Inflammatory foci, number of inflammatory foci per cm2 of tissue; real-time PCR was used to quantify relative TNF-α, IL-1β, Cox-2, and aromatase transcript levels; aromatase activity, femtomoles/μg protein/hour. Values are summarized in median (range), P values are based on Wilcoxon rank-sum test, n = 10/gp.