Table 1.

Summary of treatment protocols

GroupNo. of animalsFrom day 1From day 7After 26 wkTerminal sacrifice
A. Treatment protocol of selenomethionine experiment
130Testosterone plus 17β-estradiolControl diet (NIH-07)Testosterone plus estradiol replacement54 wk after start
230Testosterone plus 17β-estradiolSelenomethionine mixed in diet at 1.5 mg/kg (as selenium)Testosterone plus estradiol replacement54 wk after start
330Testosterone plus 17β-estradiolSelenomethionine mixed in diet at 3.0 mg/kg (as selenium)Testosterone plus estradiol replacement54 wk after start
B. Treatment protocol of α-tocopherol experiment
130Testosterone plus 17β-estradiolControl diet (NIH-07)Testosterone plus estradiol replacement54 wk after start
230Testosterone plus 17β-estradiolα-Tocopherol mixed in diet at 2,000 mg/kgTestosterone plus estradiol replacement54 wk after start
330Testosterone plus 17β-estradiolα-Tocopherol mixed in diet at 4,000 mg/kgTestosterone plus estradiol replacement54 wk after start