Table 1.

Assessment of synergy: difference in ln(NF-κB) according to prediction from main effects of the five ingredients in the combined extract compared with the effect of the combined extract

Main effects
ParameterEstimateSEMt valuePr > |t|
A. Combined extract at 3/0.3 mg/mL* vs sum of five at 3/0.3 mg/mL
Sum of five ingredients−0.420.21−1.950.075
Combined extract−2.140.23−9.17<0.001
Difference, combined extract vs sum of five−1.720.44−3.930.002
B. Combined extract at 1.5/0.15 mg/mL* vs sum of five at 3/0.3 mg/mL
Sum of five ingredients−0.420.24−1.710.112
Combined extract−1.990.27−7.49<0.001
Difference, combined extract vs sum of 5−1.570.50−3.150.008
C. Combined extract at 0.6/0.06 mg/mL* vs sum of five at 3/0.3 mg/mL
Sum of five ingredients−0.420.20−2.120.056
Combined extract−0.260.21−1.230.242
Difference, combined extract vs sum of five0.150.400.380.711
  • *Concentrations: a/b mg/mL, (a) mg/mL of each of coffee and walnut and (b) mg/mL of each of clove, thyme, and oregano.