Table 3.

The expression profiles of treatment with vorozole in rat for the estrogen-responsive genes that are predicted to be E2F1 target genes, based on Genomica analysis, and the locations of estrogen receptor and E2F binding

GeneGene accessionDescriptionVorozoleTumorPFCER-binding siteaE2F-binding sitebDistance upstream of TSS
Bub1NM_001106507Budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae)6.0217.0960.0065−2.11N/AN/AN/A
Bub1bENSRNOT00000010406Budding uninhibited by benzimidazoles 1 homolog, beta (S. cerevisiae)6.2106.9120.0021−1.63N/ATTTGGGGC346
Ccna2NM_053702Cyclin A27.2158.0620.0025−1.80N/ATTTGGCTC115
Cdc2NM_019296Cell division cycle 2, G1–S and G2–M6.2817.2280.0035−1.93ER_2352TTTAGCGC26
Cdkn3NM_001106028Cyclin-dependent kinase inhibitor 36.1747.1210.0002−1.93N/AN/AN/A
LipaNM_012732Lipase A, lysosomal acid, cholesterol esterase9.5148.7900.02551.65N/AN/AN/A
Mki67ENSRNOT00000038176Antigen identified by monoclonal antibody Ki-678.2659.3060.0054−2.06N/AN/AN/A
NaspNM_001005543Nuclear autoantigenic sperm protein (histone-binding)6.5397.3810.0037−1.80ER_101TTTCGCTC1,119
PrcpNM_001106281Prolylcarboxypeptidase (angiotensinase C)9.0438.3820.04061.58N/ATTTCGCCC10
Prss23NM_001007691Protease, serine, 239.0068.3950.00171.53ER_2571N/AN/A
Pttg1NM_022391Pituitary tumor-transforming 16.6887.2960.0166−1.52N/ATTTGGGGC137
Top2aNM_022183Topoisomerase (DNA) II alpha7.8779.2280.0010−2.55N/AN/AN/A
Tpx2NM_001107790TPX2, microtubule-associated, homolog (Xenopus laevis)7.2598.4430.0002−2.27N/AN/AN/A

Abbreviations: N/A, no observed ER- or E2F-binding site association for the estrogen-regulated gene; S. cerevisiae, Saccharomyces cerevisiae; TSS, transcription start site.

  • aEstrogen receptor–binding sites within 100 kb of an estrogen-regulated gene were determined using the published stringent (E-5) genome-wide ER-binding sites dataset of Carroll and colleagues (28). The ER-binding site identifier is based on the Carroll and colleagues numbering system.

  • bIdentified E2F-binding sites within 2 kb directly upstream of the TSS. The sequence and distance from the TSS are also noted.