Table 1.

Summary of protein expression and statistical analysis for the individual protein biomarker candidates

Protein name	Protein symbol	NCBI number	Time point (days of age)	Average expression ratio (Pirc/WT)	P	Sensitivity	Specificity	AUC	F₁–wild-type variance over time
Matrix metalloproteinase-7	MMP7	NP_036996	60	1.12	0.46	ND	ND	ND	25.7%
			90	1.38	0.04	57.1%	80.0%	0.664
			135	1.74	0.004	85.7%	80.0%	0.843
Leucine-rich alpha-2 glycoprotein	LRG1	NP_001009717	60	1.07	0.06	16.7%	100.0%	0.674	12.9%
			90	1.21	0.03	64.3%	90.0%	0.857
			135	1.43	<0.001	92.9%	90.0%	0.907
Inter-alpha trypsin inhibitor, heavy chain 4	ITIH4	NP_062242	60	1.11	0.06	50.0%	83.3%	0.701	15.0%
			90	1.14	0.03	28.6%	100.0%	0.649
			135	1.37	0.001	78.6%	90.0%	0.871
Vitronectin	VTN	NP_062029	60	1.03	0.61	8.3%	91.7%	0.504	16.2%
			90	1.12	0.001	35.7%	100.0%	0.821
			135	1.20	0.02	71.4%	90.0%	0.854
Hemopexin	HPX	NP_445770	60	1.15	0.006	50.0%	100.0%	0.708	23.3%
			90	1.26	<0.001	78.6%	100.0%	0.882
			135	1.43	0.003	85.7%	80.0%	0.792
Epidermal growth factor receptor	EGFR	NP_113695	60	0.97	0.33	8.3%	100.0%	0.632	11.8%
			90	0.87	0.002	50.0%	100.0%	0.832
			135	0.65	<0.001	100.0%	80.0%	0.939
Coagulation factor V	F5	NP_001041343	60	1.00	0.94	8.3%	100.0%	0.545	11.5%
			90	1.08	0.08	21.4%	100.0%	0.679
			135	1.24	0.007	64.3%	90.0%	0.757
Inter-alpha trypsin inhibitor, heavy chain H3	ITIH3	NP_059047	60	1.03	0.57	25.0%	91.7%	0.615	16.3%
			90	1.07	0.02	14.3%	100.0%	0.679
			135	1.05	0.34	14.3%	90.0%	0.428
Complement factor I	CFI	NP_077071	60	1.04	0.59	16.7%	91.7%	0.576	23.9%
			90	1.08	0.26	21.4%	90.0%	0.867
			135	1.13	0.24	50.0%	80.0%	0.820
Collagen, type I, alpha 1	COL1A1	NP_445756	60	1.11	0.09	8.3%	91.7%	0.309	57.6%
			90	1.1	0.11	7.1%	80.0%	0.371
			135	0.91	0.18	42.9%	70.0%	0.592
Matrix metalloproteinase 10	MMP10	NP_598198	60	1.02	0.81	8.3%	83.3%	0.462	12.0%
			90	1.03	0.30	7.1%	100.0%	0.561
			135	0.97	0.48	0.0%	90.0%	0.482
CD44 antigen	CD44	NP_037056	60	1.05	0.48	16.7%	75.0%	0.286	17.8%
			90	1.07	0.25	21.4%	90.0%	0.755
			135	0.91	0.33	7.1%	80.0%	0.672

NOTE: MMP7 expression was low in serum and was not identified at the 60-day time point.

Abbreviation: ND, not determined.