Table 1.

Effect of 1,25(OH)2 D and 25(OH)D perfusion on metastatic spread to lungs at 12 weeks

Vehicle1,25(OH)225(OH)
Percentage of animals with metastases100%100%100%
Average number of metastases per animal12.9 ± 5.45.14 ± 1.67 (P < 0.05)3.42 ± 1.22 (P < 0.002)
Relative area of metastases compared with vehicle-treated mice100%35% ± 4% (P < 0.05)25% ± 7% (P < 0.002)

NOTE: Systemic perfusion of female MMTV-PyMT mice starting at 4 weeks with 1,25(OH)2 D or 25(OH)D did not prevent appearance of tumors in lungs but reduced the size and numbers of metastases.