Table 1.

RAS gene family mutation analysis

GeneCodonPrimersPCR conditionsPCR product (bp)Analysis
KRAS125-CTGAATATAAAC TTGTGGTAGTTGGACCT-394°C for 55 seconds, 58°C for 45 seconds, and 72°C for 45 seconds157Sequence analysis
5-TCAAAGAATGGT CCTGGACC-3
HRAS125-AGACCCTGTAGG AGGACCC-394°C for 55 seconds, 62°C for 50 seconds, and 72°C for 50 seconds312Sequence analysis + RFLP (AVA II)
5-GGTGCTGAGACG AGGGACT-3
NRAS125-AACTGGTGGTGG T TGGACCA-394°C for 55 seconds, 57°C for 50 seconds, and 72°C for 40 seconds83Sequence analysis
5-ATATTCATCTAC AAAGTGGT-3