Table 1.

Effect of aspirin dosing regimens on the modulation of markers of proliferation, apoptosis, and inflammation in AOM-induced rat colonic adenocarcinoma

Inflammatory cytokine levels (pg/mg protein) mean ± SEM, N = 6–9
Experimental groupsFrequency of dose administrationPCNA PIp21 expression IHC score (1–12)p53 expression IHC score (1–12)Caspase-3 expression IHC score (1–12)IL1βIL6IL12IL10PGE2
1Control diet56.7 ± 5.20.5 ± 0.212.1 ± 0.303.1 ± 0.512,673 ± 95557 ± 36273 ± 1842 ± 4.72,278 ± 88
2AspirinContinuous38.5 ± 3.74.7 ± 0.523.8 ± 0.66.5 ± 1.11,556 ± 58322 ± 23185 ± 1469 ± 5.81,477 ± 58
700 ppmP < 0.006P < 0.0001P < 0.014P < 0.008P < 0.0001P < 0.005P < 0.0009P < 0.0012P < 0.0001
3AspirinContinuous27.7 ± 3.27.7 ± 1.14.5 ± 0.98.4 ± 1.31,163 ± 42247 ± 17134 ± 1184 ± 7.1734 ± 43
1,400 ppmP < 0.0003P < 0.0001P < 0.016P < 0.002P < 0.0001P < 0.0001P < 0.0001P < 0.0001P < 0.0001
4Aspirin1 week on/42.3 ± 4.13.6 ± 0.83.9 ± 0.75.9 ± 0.91,673 ± 67356 ± 29202 ± 1662 ± 5.21,672 ± 62
700 ppm1 week offP < 0.023P < 0.002P < 0.02P < 0.01P < 0.0001P < 0.0005P < 0.007P < 0.0065P < 0.0001
5Aspirin1 week on/33.7 ± 2.86.9 ± 1.25.3 ± 1.07.7 ± 1.11,338 ± 48298 ± 23168 ± 9.873 ± 6.11,073 ± 48
1,400 ppm1 week offP < 0.0015P < 0.0001P < 0.007P < 0.0015P < 0.0001P < 0.0001P < 0.0001P < 0.0005P < 0.0001
6Aspirin3 weeks on/47.3 ± 4.82.9 ± 0.91.8 ± 0.94.2 ± 0.82,132 ± 63429 ± 27234 ± 1854 ± 4.21,748 ± 69
700 ppm3-weeks offP = 0.1P < 0.016P = 0.38P = 0.13P < 0.0002P < 0.009P = 0.08P < 0.043P < 0.0002
7Aspirin3 weeks on/37.7 ± 3.53.5 ± 0.72.8 ± 0.64.5 ± 0.71,858 ± 66377 ± 21200 ± 1260 ± 4.71,487 ± 57
1,400 ppm3 weeks offP < 0.005P < 0.0013P = 0.15P = 0.063P < 0.0001P < 0.0007P < 0.004P < 0.009P < 0.0001
8Aspirin3 weeks on/32.0 ± 4.16.2 ± 1.34.4 ± 0.88.6 ± 1.61,587 ± 49311 ± 22172 ± 1374 ± 6.3845 ± 39
2,800 ppm3 weeks offP < 0.0016P < 0.001P < 0.011P < 0.005P < 0.0001P < 0.0001P < 0.0001P < 0.0005P < 0.0001
  • NOTE: Mean ± SEM (N = 6–9). Values are significantly different from control diet group per t test with Welch correction.